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Interpreting Perturb-seq data remains challenging because single-cell RNA sequencing often misses transcripts from lowly expressed genes, producing zeros that can reflect either true knockdown or simple dropout. This ambiguity blurs the distinction between effective perturbations and escaped cells, making it difficult to assess gRNA performance, especially in large screens where Read More

1. The Alignment/Registration Problem in Spatial Imaging In spatial imaging, researchers often analyze more than one representation of the same tissue. An H&E image provides structural context, while an IF image highlights specific markers. Many workflows even use a series of adjacent sections, where each section serves a different purpose. Read More

Astrocyte segmentation is challenging because these cells do not have simple shapes, are densely packed, and often intertwine with each other. Mapping them in brain images is notoriously difficult – signals blur, noise dominates, and current cell segmentation algorithms often fail, especially in injured tissues. Scientists at BioTuring and UC Read More

1. The Target Identification Problem in IBD The core challenge in Inflammatory Bowel Disease (IBD) research is tissue heterogeneity. Inflammation affects different regions of the intestine and different cell types in distinct ways, making it difficult to interpret gene expression changes. Without knowing which cell type expresses a gene and Read More

Colorectal cancer (CRC) is the third most prevalent cancer and the second leading cause of cancer-related deaths worldwide [1]. Progressive plasticity refers to the ability of cancerous cells to adapt their state, phenotype, and molecular characteristics in response to environmental stimuli. This plasticity significantly contributes to the metastasis of cancerous Read More

This blog highlights a case study [1] using single-cell RNA sequencing (scRNA-seq) to investigate associations between prurigo nodularis (PN) and skin cancer risk, featuring insights via BioTuring‘s  BBrowserX and Talk2Data platforms. A mini-webinar is available on the BioTuring Team’s channel. What is prurigo nodularis? Read More