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  • Species: human
  • Number of cells: 2707
  • Number of downloads: 63
  • Study size: 36MB
  • Uploaded at: Jun 19, 2020


Human germinal center transcriptional programs are de-synchronized in B cell lymphoma

Pierre Milpied, Iñaki Cervera-Marzal, Marie-Laure Mollichella, Bruno Tesson, Gabriel Brisou, Alexandra Traverse-Glehen, Gilles Salles, Lionel Spinelli, Bertrand Nadel

Most adult B cell lymphomas originate from germinal center (GC) B cells, but it is unclear to what extent B cells in overt lymphoma retain the functional dynamics of GC B cells or are blocked at a particular stage of the GC reaction. Here we used integrative single-cell analysis of phenotype, gene expression and variable-region sequence of the immunoglobulin heavy-chain locus to track the characteristic human GC B cell program in follicular lymphoma B cells. By modeling the cyclic continuum of GC B cell transitional states, we identified characteristic patterns of synchronously expressed gene clusters. GC-specific gene-expression synchrony was lost in single lymphoma B cells. However, distinct follicular lymphoma–specific cell states co-existed within single patient biopsies. Our data show that lymphoma B cells are not blocked in a GC B cell state but might adopt new dynamic modes of functional diversity, which opens the possibility of novel definitions of lymphoma identity.

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