Try our BETA version of Cell type Prediction now for free

Contact us
  • Species: mouse
  • Number of cells: 1845
  • Number of downloads: 10
  • Study size: 14MB
  • Uploaded at: Jul 15, 2021

CharacterizationBrainArea postrema

Area Postrema Cell Types that Mediate Nausea- Associated Behaviors (All Neurons - Exon and Intron)

Chuchu Zhang, Judith A Kaye, Zerong Cai, Yandan Wang, Sara L Prescott, Stephen D Liberles

Nausea, the unpleasant sensation of visceral malaise, remains a mysterious process. The area postrema is implicated in some nausea responses and is anatomically privileged to detect blood-borne signals. To investigate nausea mechanisms, we built an area postrema cell atlas through single-nucleus RNA sequencing, revealing a few neuron types. Using mouse genetic tools for cell-specific manipulation, we discovered excitatory neurons that induce nausea-related behaviors, with one neuron type mediating aversion imposed by multiple poisons. Nausea-associated responses to agonists of identified area postrema receptors were observed and suppressed by targeted cell ablation and/or gene knockout. Anatomical mapping revealed a distributed network of long-range excitatory but not inhibitory projections with subtype-specific patterning. These studies reveal the basic organization of area postrema nausea circuitry and provide a framework toward understanding and therapeutically controlling nausea.

Back to datasets