Which dataset
would you like to
analyze in BBrowser?

Species: mouse
Number of cells: 3488
Study size: 802MB

brain 

Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

Amit Zeisel, Ana B. Munoz-Manchado, Simone Codeluppi, Peter L÷nnerberg, Gioele La Manno, Anna JurΘus, Sueli Marques, Hermany Munguba, Liqun He, Christer Betsholtz, Charlotte Rolny, Gonτalo Castelo-Branco, Jens Hjerling-Leffler, Sten Linnarsson

The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature. Here, we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified numerous marker genes, which allowed alignment with known cell types, morphology, and location. We found a layer I interneuron expressing Pax6 and a distinct postmitotic oligodendrocyte subclass marked by Itpr2. Across the diversity of cortical cell types, transcription factors formed a complex, layered regulatory code, suggesting a mechanism for the maintenance of adult cell type identity.

Download bbrowser to analyze now

About 1 datasets