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10X Spatial Gene Expression Datasets - Normal Human Prostate (FFPE)
Spatial Gene Expression Dataset by Space Ranger 1.3.0
5 µm section from human prostate. FFPE tissue purchased from Indivumed Human Tissue Specimens (Indivumed).
Libraries were prepared following the Visium Spatial Gene Expression Reagent Kits for FFPE User Guide (CG000407 Rev A).
Sequenced on Illumina NovaSeq with dual index set SI-TS-D2, approximately 27,893 reads per spot, 28bp read 1 (16bp Visium spatial barcode, 12bp UMI), 50bp read2 (transcript), 10bp i7 sample barcode and 10bp i5 sample barcode.
FASTQ data were analyzed with Space Ranger 1.3. 2539 spots detected under tissue.