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Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq

Itay Tirosh, Benjamin Izar, Sanjay M. Prakadan, Marc H. Wadsworth II, Daniel Treacy, John J. Trombetta, Asaf Rotem, Christopher Rodman, Christine Lian, George Murphy, Mohammad Fallahi-Sichani, Ken Dutton-Regester, Jia-Ren Lin, Ofir Cohen, Parin Shah, Diana Lu, Alex S. Genshaft, Travis K. Hughes, Carly G. K. Ziegler, Samuel W. Kazer, Aleth Gaillard, Kellie E. Kolb, Alexandra-Chloé Villani, Cory M. Johannessen, Aleksandr Y. Andreev, Eliezer M. Van Allen, Monica Bertagnolli, Peter K. Sorger, Ryan J. Sullivan, Keith T. Flaherty, Dennie T. Frederick, Judit Jané-Valbuena, Charles H. Yoon, Orit Rozenblatt-Rosen, Alex K. Shalek, Aviv Regev, Levi A. Garraway

Tumors harbor multiple cell types that are thought to play a role in the development of resistance to drug treatments. Tirosh et al. used single-cell sequencing to investigate the distribution of these differing genetic profiles within melanomas. Many cells harbored heterogeneous genetic programs that reflected two different states of genetic expression, one of which was linked to resistance development. Following drug treatment, the resistance-linked expression state was found at a much higher level. Furthermore, the environment of the melanoma cells affected their gene expression programs.

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Species: human
Number of cells: 4645
Number of downloads: 164
Study size: 285MB
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Immunology 
Immunotherapy 
skin 
tumour-site 
melanoma