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Single-cell atlas of colonic CD8+ T cells in ulcerative colitis (cite-seq)

Corridoni, Daniele and Antanaviciute, Agne and Gupta, Tarun and Fawkner-Corbett, David and Aulicino, Anna and Jagielowicz, Marta and Parikh, Kaushal and Repapi, Emmanouela and Taylor, Steve and Ishikawa, Dai and others

Colonic antigen-experienced lymphocytes such as tissue-resident memory CD8+ T cells can respond rapidly to repeated antigen exposure. However, their cellular phenotypes and the mechanisms by which they drive immune regulation and inflammation remain unclear. Here we compiled an unbiased atlas of human colonic CD8+ T cells in health and ulcerative colitis (UC) using single-cell transcriptomics with T-cell receptor repertoire analysis and mass cytometry. We reveal extensive heterogeneity in CD8+ T-cell composition, including expanded effector and post-effector terminally differentiated CD8+ T cells. While UC-associated CD8+ effector T cells can trigger tissue destruction and produce tumor necrosis factor (TNF)-α, post-effector cells acquire innate signatures to adopt regulatory functions that may mitigate excessive inflammation. Thus, we identify colonic CD8+ T-cell phenotypes in health and UC, define their clonal relationships and characterize terminally differentiated dysfunctional UC CD8+ T cells expressing IL-26, which attenuate acute colitis in a humanized IL-26 transgenic mouse model.

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Species: human
Number of cells: 11704
Number of downloads: 94
Study size: 185MB
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ulcerative colitis