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Regenerative potential of prostate luminal cells revealed by single-cell analysis (mouse)

Wouter R Karthaus, Matan Hofree, Danielle Choi, Eliot L Linton, Mesruh Turkekul, Alborz Bejnood, Brett Carver, Anuradha Gopalan, Wassim Abida, Vincent Laudone, Moshe Biton, Ojasvi Chaudhary, Tianhao Xu, Ignas Masilionis, Katia Manova, Linas Mazutis, Dana Pe'er, Aviv Regev, Charles L Sawyers

Androgen deprivation is the cornerstone of prostate cancer treatment. It results in involution of the normal gland to ~90% of its original size because of the loss of luminal cells. The prostate regenerates when androgen is restored, a process postulated to involve stem cells. Using single-cell RNA sequencing, we identified a rare luminal population in the mouse prostate that expresses stemlike genes (Sca1+ and Psca+) and a large population of differentiated cells (Nkx3.1+, Pbsn+). In organoids and in mice, both populations contribute equally to prostate regeneration, partly through androgen-driven expression of growth factors (Nrg2, Rspo3) by mesenchymal cells acting in a paracrine fashion on luminal cells. Analysis of human prostate tissue revealed similar differentiated and stemlike luminal subpopulations that likewise acquire enhanced regenerative potential after androgen ablation. We propose that prostate regeneration is driven by nearly all persisting luminal cells, not just by rare stem cells.

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Species: mouse
Number of cells: 87187
Number of downloads: 23
Study size: 3GB
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reproductive system