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Single-cell transcriptomics identifies multiple pathways underlying antitumor function of TCR- and CD8αβ-engineered human CD4+ T cells

Jan A. Rath, Gagan Bajwa, Benoit Carreres, Elisabeth Hoyer, Isabelle Gruber, Melisa A. Martínez-Paniagua, Yi-Ru Yu, Nazila Nouraee, Fatemeh Sadeghi, Mengfen Wu, Tao Wang, Michael Hebeisen, Nathalie Rufer, Navin Varadarajan, Ping-Chih Ho, Malcolm K. Brenner, David Gfeller, Caroline Arber

Transgenic coexpression of a class I–restricted tumor antigen–specific T cell receptor (TCR) and CD8αβ (TCR8) redirects antigen specificity of CD4+ T cells. Reinforcement of biophysical properties and early TCR signaling explain how redirected CD4+ T cells recognize target cells, but the transcriptional basis for their acquired antitumor function remains elusive. We, therefore, interrogated redirected human CD4+ and CD8+ T cells by single-cell RNA sequencing and characterized them experimentally in bulk and single-cell assays and a mouse xenograft model. TCR8 expression enhanced CD8+ T cell function and preserved less differentiated CD4+ and CD8+ T cells after tumor challenge. TCR8+CD4+ T cells were most potent by activating multiple transcriptional programs associated with enhanced antitumor function. We found sustained activation of cytotoxicity, costimulation, oxidative phosphorylation– and proliferation-related genes, and simultaneously reduced differentiation and exhaustion. Our study identifies molecular features of TCR8 expression that can guide the development of enhanced immunotherapies.

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Species: human
Number of cells: 25474
Number of downloads: 39
Study size: 515MB
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Immunology 
Immunotherapy