Which dataset
would you like to
analyze in BBrowser?

Landscape of intercellular crosstalk in healthy and NASH liver revealed by single-cell secretome gene analysis

Xiong, Xuelian and Kuang, Henry and Ansari, Sahar and Liu, Tongyu and Gong, Jianke and Wang, Shuai and Zhao, Xu-Yun and Ji, Yewei and Li, Chuan and Guo, Liang and others

Cell-cell communication via ligand-receptor signaling is a fundamental feature of complex organs. Despite this, the global landscape of intercellular signaling in mammalian liver has not been elucidated. Here we perform single-cell RNA sequencing on non-parenchymal cells isolated from healthy and NASH mouse livers. Secretome gene analysis revealed a highly connected network of intrahepatic signaling and disruption of vascular signaling in NASH. We uncovered the emergence of NASH-associated macrophages (NAM), which are marked by high expression of Triggering Receptor Expressed on Myeloid Cells 2 (Trem2), as a feature of mouse and human NASH that is linked to disease severity and highly responsive to pharmacological and dietary interventions. Finally, hepatic stellate cells (HSC) serve as a hub of intrahepatic signaling via HSC-derived stellakines and their responsiveness to vasoactive hormones. These results provide unprecedented insights into the landscape of intercellular crosstalk and reprogramming of liver cells in health and disease.

Download bbrowser to analyze now

Species: mouse
Number of cells: 33168
Number of downloads: 53
Study size: 1017MB
Uploaded at:

Immunology 
liver